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流式细胞仪检测胶原诱导的关节炎大鼠γδT细胞胞内TNF-α和IL-10的表达 被引量：1

Detection of intracellular expressions of TNF-α and IL-10 in γδT lymphocytes of rat with collagen- induced arthritis by flow cytometry
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摘要 目的研究牛Ⅱ型胶原诱导的关节炎模型(CIA)大鼠外周血中γδT细胞胞内TNF-α和IL-10的表达情况,探讨其在CIA中作用的分子机制。方法于Wistar大鼠背部及尾根部多点皮内注射牛Ⅱ型胶原乳剂制作CIA模型。运用流式细胞仪,通过直接荧光标记的抗TNF-α和抗IL-10抗体来检测单个细胞水平γδT细胞胞内细胞因子的表达。结果与正常大鼠相比,CIA模型大鼠外周血中γδT细胞所占淋巴细胞的百分比显著下降(P<0.01),γδT细胞胞内TNF-α(P<0.01)和IL-10(P<0.01)的表达均明显降低。结论γδT细胞在CIA中其前炎症细胞因子表达下调,可能γδT细胞在CIA的发病中不起致病作用。 Objective To detect the expressions of TNF-α and IL-10 in γδT lymphocytes in the rat model of collagen-induced arthritis (CIA) in order to discuss the possible molecular mechanism of γδT cells in CIA. Methods The experimental rat model of CIA was constructed by injection of the emulsion of bovine type Ⅱ collagen. The intracytoplasmic TNF-α and IL-10 were detected by flow cytometry and direct-labeled antibody, respectively. Results The percentage of γδT lymphocytes (P < 0.01) in the total lymphocytes in the rat model of CIA and the expressions of intracellular TNF-α ( P < 0.01) and IL-10 ( P < 0.01) of γδT lymphocytes were obviously decreased. Conclusion The γδT lymphocytes may not play a pathologic role in the mechanism of CIA for the down-regulation of the pro-inflammatory cytokines.
作者 曾克勤 胡永红 张明敏 赖先阳 张玮琛
机构地区 华中科技大学同济医学院附属同济医院中西医结合科
出处 《免疫学杂志》 CAS CSCD 北大核心 2004年第z1期137-139,142,共4页 Immunological Journal
基金 国家自然科学基金资助项目(30070961)
关键词 流式细胞术 胶原诱导的关节炎 ΓΔT细胞 细胞因子 类风湿关节炎 <Keyword>ow cytometry Collagen induced arthritis γδT lymphocyte Cytokine Rheumatoid arthritis
分类号 R684 [医药卫生—骨科学]
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参考文献14

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二级参考文献12

共引文献27

同被引文献14

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免疫学杂志
2004年 第z1期

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