复发性自然流产患者绒毛滋养细胞FasL的表达

Expression of FasL on Trophoblasts of Recurrent Spontaneous Abortion

目的探讨复发性自然流产患者(RSA)绒毛滋养细胞FasL的表达与正常者有无差异。方法应用免疫组化和RT-PCR法,检测RSA患者和正常早孕妇女的绒毛滋养细胞FasL在蛋白水平和mRNA水平的表达,并进行图象分析和比较。结果免疫组化结果显示,绒毛合体滋养细胞和细胞滋养细胞的胞浆和胞膜上均有FasL表达,FasL在2组的定位无差异,但正常组绒毛滋养细胞FasL表达(PU=18.03±4.69)强于RSA组(PU=13.59±3.73),P<0.050。RT-PCR结果表明RSA组绒毛组织FasL在mRNA水平的表达为0.18±0.05,低于正常组0.27±0.06,差异有显著性意义(P<0.01)。结论不明原因RSA患者的绒毛滋养细胞FasL表达弱于正常者,滋养细胞FasL的表达减少可能在复发性自然流产中起重要作用。

Objective To investigate the expressions of FasL on trophoblast of recurrent spontaneous abortion patients and normal pregnant women. Methods The immunohistochemistry and RT-PCR were applied to investigate and compare the expressions of FasL on trophoblast of recurrent spontaneous abortion (RSA) patients and normal early pregnant women. Results The expressions of FasL in cytomembrane or cy-toplasma of cytotrophoblast cells and syncytiotrophoblast were detected by immunohistochemistry staining. The localization of FasL in the two groups was similar, but the expression level of FasL in RSA group (PU = 18.03 ± 4.69) was lower than that in the normal group (PU = 13.59 ± 3.73) (P<0.05). Significant difference of the FasL mRN A expressions in RSA group (0.18 ± 0.05) and normal group (0.27 ± 0.06) were found (P < 0.01). Conclusion The FasL expressions on trophoblast of RSA patients are significant lower than that of normal control group. The decrease of FasL expression may play an important role in the etiology of RSA.