摘要
采用SDS 聚丙烯酰胺凝胶电泳法 ,对 2 0 0 2～ 2 0 0 3年间从新疆各主要植棉区采集到的 31个棉花枯萎病菌菌株及 3号、7号和 8号生理小种的标准菌株进行了可溶性蛋白质谱分析。结果表明 ,其不同生理小种的可溶性蛋白质谱存在明显差异 ,同一小种不同菌株的谱带一致性较高。由此说明可溶性蛋白凝胶电泳对棉花枯萎病菌生理小种的鉴定有重要参考价值。
The soluble protein bands of 31 isolates from diseased cotton stalks of main cotton production region in Xinjiang and different standard physiologic races (3、7、8)were analysed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The result of the experiment showed that the difference of protein bands between physiologic races of cotton wilt .fusarium was obvious, the identity of protein bands among different strains of the same physiologic race was considerable high. These identified that the electrophoretic analysis of soluble protein was of consult value in identification of physiologic races of cotton-wilt fusarium.
出处
《石河子大学学报（自然科学版）》
CAS
2004年第z1期49-51,共3页
Journal of Shihezi University(Natural Science)
基金
新疆兵团绿洲生态农业重点实验室首批开放课题 (2 0 0 315 )
关键词
棉花
枯萎病菌
生理小种
可溶性蛋白
质谱分析
cotton
cotton-wilt pathogen
physiologic race
soluble protein
analysis
