470例真菌感染的分离鉴定及耐药性分析

Separation and identification of 470 strains of fungi and analysis of drug resistance

目的了解念珠菌感染和耐药情况,为临庆用药提供参考。方法从标本中分离真菌后,用API系统20CAUX进行鉴定,用AT Fungus药敏条测定真菌对6种常用抗真菌药的体外敏感性。结果 临床念珠菌感染以呼吸道感染为主占48.9％;菌种分布以白色念珠菌占的比例最高为61.9％,其次为热带念珠菌(14.9％)、光滑念珠菌(97.4％);在药敏结果中,以氟胞嘧啶、两性霉素B、制霉素的作用最强,益康唑的作用最差。结论临床上念珠菌感染以白色念珠菌引起的呼吸道感染为主,唑类药物真菌的检测和耐药性监测工作,以指导临床合理用药。

ObjictiveTo understand the Canidida sppinfection and the antifungal agents resistance in our hospital for providing clinical reference.MethodsAfter the fungi were isolated from clinical specimen,using API system 20CAUX to performe identification,the ersis-tance to six kinds of antifungal agents was tested by ATB fungus drug susceptibility strip.Results Of 470 strains of clinical canidida spp infectionj,the respiratory infection accounted for 48.9%.The prevalence rate of Canidida spp infection was:C albicans(61.9%),C tropicalis (14.9%),C glabrata(7.4%).Results of drug susceptibility test showed that the susceptibilities to 5-fluorocystine,Ampho-tricin B,Nystatin were the highest,and Econazol was the lowest.Conclusions The prevalance rate of the respiratory infection due to C albicans was the highest in canidida spp infection.The resistance rate of axole drug was higher thab other antifungal agents.The isolation of fungi and the antifungal resistivity test should be strengthed in clinical laboratory in order to quide clinical selection of the res-sonable antibiotic.