期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

半乳糖修饰大孔壳聚糖支架的制备及表征 被引量：2

The preparation and characterization of porous galactosylated chitosan scaffolds
下载PDF
导出
摘要 生物人工肝反应器中肝细胞的数量和功能是能否有效替代已衰竭肝脏功能的关键,与此紧密相关的是细胞支架材料结构和性能的优化.采用冷冻干燥法,制备了大孔壳聚糖支架,孔隙率在90%以上,平均孔径在100～200μm之间.以肝细胞表面去唾液酸糖蛋白受体(asialoglycoprotein receptor,ASGPR)的特异性配体-半乳糖,对材料表面进行糖基化修饰,制备了半乳糖基修饰的大孔壳聚糖支架,肝细胞在其上生长状况良好,细胞培养密度高,为高密度培养肝细胞提供了一种性能优良的支架材料.
作者 包志明 潘继伦 吴晨 俞耀庭
机构地区 南开大学
出处 《功能材料》 EI CAS CSCD 北大核心 2004年第z1期2454-2456,共3页 Journal of Functional Materials
基金 天津市自然科学基金资助项目(033608011)
关键词 壳聚糖 半乳糖 冷冻干燥法 生物人工肝
分类号 O636 [理学—高分子化学]
  • 相关文献

参考文献8

  • 1[1]Hayes, Peter C. Lee, Alistair. What progress with artificial livers. [J]. The Lancet. 2001, 358(9290): 1286-1287.
  • 2[2]Kaneko M, Fukuda J, Ijima H, et. al. Development of hybrid artificial liver support system using spheroid culture and application to warm ischemic liver failure in dog and pig as a preclinical test. [J]. Materials Science and Engineering: C.1998, 6(4): 245-248.
  • 3[3]Madihally SV, Matthew HWT. Porous chitosan scaffolds for tissue engineering. [J]. Biomaterials, 1994, 20:1133-42.
  • 4[4]Chandy F, Sharma P. Chitosan as a biomaterial. [J].Biomater ArtifCells ArtifOrg, 1990, 18: 1-24.
  • 5[5]Park TG. Perfusion culture of hepatocytes within galactose derivatized biodegradable poly (lactide-co-glycolide) scaffolds prepared by gas foaming of effervescent salts. [J]. J Biomed Mater Res, 2002, 59: 127-135.
  • 6[6]Shen F, Cui YL, Yang LF, et al. A study on the fabrication of porous chitosan/gelatin network scaffold for tissue engineering. [J]. Polym Int, 2000, 49:1596-1599.
  • 7[7]Park IK, Yang J, Jeong HJ, et al. galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment.[J]. Biomaterials. 2003, 24:2331-2337.
  • 8[8]Seglen PO. Preparetion of rat liver cells. [J]. Exp Cell Res.1972, 74: 450-454.

同被引文献29

  • 1龙仕和,李明生,靳冬武,马忠仁,冯玉萍.天然高分子细胞培养基质的研究进展[J].高分子通报,2015(2):40-46. 被引量：2
  • 2SINGH R, SINGH S, TRIMUKHE K D, et al. Lignin-carbohydrate complexes from sugarcane bagasse: Preparation, purification, and characterization [ J ]. Carbohydrate Polymers ,2005,62 ( 1 ) :57-66.
  • 3KOSHIJIMA T, WATANABE T. Structure and properties of the lignin-carbohydrate complex polymer as an ampbipathic substance[ J]. American Chemical Society, 1989,397 (2) : 11-28.
  • 4小林一清.7クロモノマーの重合を経山する「クラスター型」の高分子配糖体の合成[C]//Abstracts of tbe 42nd annua] meeting of the Japan Wood Society, Nagoya, 1992:341.
  • 5ERAKOV1C S, VELJOVIC D, DIOUF P. Electrophoretic deposition of biocomposite lignin/hydroxyapatite coatings on titanium[ J l. International Joural of Chemical Reaction Engineering,2009,7 ( 1 ) :1-21.
  • 6MANSUR H S, MANSUR A A P, BICALLHO S C M. Lignin-hydroxyapatite/tricalcium phosphate biocomposites: SEM/EDX and FT-IR charaeterization [ J ]. Key Engineering Materials,2005 ( 284/285/286 ) : 745-748.
  • 7MARTINEZ M M, PACHECO B A,VARGAS V M. Histological evaluation of the biocompatibility and bioconduction of a hydroxyapatite-lignin compound inserted in rabbits' shinbones. Revista MVZ Cordoba,2009,14 ( 1 ) :1624-1632.
  • 8PARK I K, YANG J, JEONG H J, et al. Galactosylated chitosan as a synthetic extracellular matrix for hepatoeytes attachment [ J ]. Biomaterials, 2003,24( 13 ) :2331-2337.
  • 9BJORKMAN A. Studies on findy divided wood. Part 3. Extraction of lignin-carbohydrate complexes with neutral solvent [ J ] Svensk Papperstding, 1957,60 : 243-251.
  • 10HSU Teng-chieh, GUO Gia-Luen, CHEN Wen-hua, et al. Effect of dilute acid pretreatment of rice straw on structural properties and enzymatic hydrolusis[ J ]. Bioresource Technology ,2010,101 ( 13 ) :4907-4913.

引证文献2

二级引证文献4

功能材料
2004年 第z1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部