急性冠状动脉综合征患者外周血单核细胞环氧合酶2mRNA表达及阿托伐他汀的作用

Atorvastatin Reduces the Expression of Cyclooxygenase-2 mRNA in Peripheral Blood Monocytes From Pateints with Acute Coronary Syndrome

为检测急性冠状动脉综合征患者外周血单核细胞中环氧合酶 2表达及阿托伐他汀的影响 ,探讨环氧合酶 2在动脉粥样硬化中的作用和他汀类药物的抗炎机制。采用逆转录聚合酶链反应半定量的方法 ,观察 4 2例急性冠状动脉综合征患者和 18例健康自愿者外周血单核细胞环氧合酶 2的表达 ,同时观察急性冠状动脉综合征组单核细胞在不同浓度阿托伐他汀 (0～ 10 μmol L)干预 2 4h后环氧合酶 2表达的变化。结果发现 ,急性冠状动脉综合征患者外周血单核细胞中环氧合酶 2的表达明显高于健康对照组 (1.6 4± 0 .2 5比 0 .5 9± 0 .2 1,P <0 .0 5 )。阿托伐他汀在体外明显抑制急性冠状动脉综合征患者外周血单核细胞环氧合酶 2的表达 (P <0 .0 5 ) ,且呈浓度依赖性。结果提示 ,环氧合酶 2在动脉粥样硬化炎症中起作用 ,阿托伐他汀明显降低急性冠状动脉综合征患者外周血单核细胞环氧合酶 2的表达 ,环氧合酶 2可能是阿托伐他汀发挥降脂外抗炎作用的靶点之一。

Aim To observe the expression of cyclooxygenase-2 (COX-2) mRNA in peripheral blood monocytes from patients with acute coronary syndrome (ACS), and the effects of atorvastatin on it. Methods Expression of COX-2 mRNA in peripheral blood monocytes from 18 normal control subjects and 42 patients with acute coronary syndrome were analyzed by reverse transcription -polymerase chain reaction (RT-PCR), and the monocytes from patients had been incubated with atorvastatin (0～10 μmol/L)in vitro. Results Expression of COX-2 mRNA significantly increased in peripheral blood monocytes from the patients with ACS compared with those from normal control subjects (1.64±0.25 vs 0.59±0.21, P<0.05). Atorvastatin significantly reduced the expression of COX-2 mRNA in peripheral blood monocytes from patients with ACS (P<0.05), and the reduction is relative to the concentration of atorvastatin. Conclusions These findings suggest that COX-2 may play an important role on the inflammation in atherosclerosis. Atorvastatin may have anti-inflammatory effects which are related to its effect on COX-2.