载脂蛋白E基因多态性与冠心病的关系

The Study of Relationship Between Apolipoprotein E Polymorphism and Coronary Heart Disease

目的 研究载脂蛋白E(ApoE)基因多态性与冠心病发病的关系。 方法 选择 90例冠心病患者 (CHD组 )及 90例非冠心病患者 (对照组 ) ,应用聚合酶链反应法 (PCR) ,HhaI内切酶消化法确定ApoE基因多态性。 结果 共检测出 5种基因型 ,分别是 :E2 /3、E3 /3、E2 /4、E3 /4、E4/4。冠心病患者的E3 /4基因型频率和ε4等位基因频率分别为 2 1.1%和 16.1% ,明显高于对照组 (P <0 .0 5 ) ;对照组ε4等位基因频率为 5 % ,远低于西方人群。 结论 ApoE基因多态性同冠心病发病有关 ,E4等位基因是影响冠心病发病的重要遗传因素之一。

Objective To study the relationship between apolipoprotein E polymorphism and coronary heart disease(CHD). Method We studied 90 patients with CHD and 90 control subjects without symptoms and sign of CHD. Polymorphism of apolipoprotein E alleles were measured by polymerase chain reaction (PCR),HhaI anzyme digestion and polyacrylamide gel electrophoresis. Results 5 genotypes,including E2/3,E3/3,E2/4,E3/4 and E4/4 were examined. The frequencies of apolipoprotein ε4 allele and E3/4 genotype(16.1% and 21.1% respectively) were obviously higher in CHD group than in control group (P<0.05). The frequency of apolipoprotein ε4 allele (5%) in control group was lower than that of people in western countries. Conclusion The apolipoprotein E polymorphism is related to the occurrence of coronary heart disease;apolipoprotein E4 allele is one of the most important hereditary factor for the occurrence of CHD.