葡萄卷叶病毒RT-PCR检测技术研究

Studies on RT-PCR Detection Technology of GLRaV Ⅲ

以葡萄叶片和皮层为材料,对植物总RNA和dsRNA提取方法进行了研究,从中筛选出了提取葡萄总RNA和dsRNA的适合方法。建立了GLRaV RT-PCR的有效体系,在有效体系基础上,通过研究RT-PCR主要成分对RT-PCR的影响,确立了GLRaVRT-PCR的优化体系。

Using grapevine leaves and cortexes as materials, total RNA was extracted using three methods ,and compared these methods in these paper. The fittest methods for Grapevine were screened out. PCR amplification conditions were optimized. Based on the effectual RT-PCR detection system of Grapevine leafroll virus Ⅲ. Concentration of RT reaction and PCR reaction primary ingredients were optimized and decrease correctly.