深圳市艾滋病分子流行病学和HIV膜蛋白基因变异与感染时间相关性分析

MOLECULAR EPIDEMIOLOGY ANALYSIS OF HIV IN SHENZHEN AND REPLICATION AN ALYSIS BETWEEN HIV-env GENE VARIATION AND EPIDEMIC TIME.

目的 :对深圳市 HIV感染流行趋势分析 ,为深圳市艾滋病防治策略提供依据。方法 :对 1992～ 2 0 0 0年深圳市HIV血清学监测质料及应用套式 PCR (nested- PCR)对深圳市 5 5例 HIV感染者 / AIDS病人的外周血的单核细胞进行扩增 ,获得了 HIV- 1膜蛋白 (env)基因的核酸片段 ,并对其 C2 - C3区进行测定和分析。结果 :深圳市疫情快速增长趋势明显 ;传播方式以性为主 (46 .0 % )其它各种 HIV传播途径均有发现 ;有 B′、 B、 C、 E亚型 HIV- 1毒株序列 ,各亚型间的基因离散率为 6 .31%、 7.95 %、 3.0 9%、 8.92 % ,推算其流行时间为 6 ,8,3,9年 ,深圳流行株以 E、 B为主 ,且 AIDS病人抗原性变异较高于 HIV感染者。结论 :监测数据可反映深圳市 HIV感染流行主流 ,可预测流行趋势。

Objective:To provide the fresh evidence for the policy-maker t o analysis the trends of HIV epidemic in Shenzhen.Methods:DNA fragments of HIV-1 env gene were amplified by nested-PCR from uncultured peripheral blood m o nonuclear cells (PBMCS) obtained from 55 HIV infected individuals and AIDS patie nts found in Shenzhen city.The C2-C3 region of them were sequenced.Re sults:Sequence analysis showed that there were four HIV-1 subtypes,B′ ,B,C,E, with 6.31%,7.95%,3.09% and 8.92% gene divergence inside each subtypes in S henz hen.These results suggested that epidemic time were 6,8,3,9,and compared w ith epidemiology were close.The main epidemic subtypes were E and B strains ,AI DS patients antigenicity variation was higer for HIV infected individuals.Con clusion:The date of surveillance can reflect the main trend of HIV epid emic in Shenzhen and can predict the trend.