血管内皮型一氧化氮合酶基因Glu298 Asp多态性与老年脑梗死的相关性研究

Association of Glu298 Asp polymorphism of the endothelial nitric oxide synthase gene with cerebral infarction in aged people

目的 通过病例对照研究 ,了解中国老年人群血管内皮型一氧化氮合酶 (eNOS)基因目的 通过病例对照研究 ,了解中国老年人群血管内皮型一氧化氮合酶 (eNOS)基因Glu2 98Asp多态性的分布 ,分别探讨其与老年脑梗死及血脂以及一氧化氮等脑梗死危险因素的关系。 方法对门诊及住院中确诊的 4 0例老年脑梗死和 16 9例性别、年龄相匹配的老人 ,测量他们的身高、体重及座位血压 ,并测定他们的空腹血脂、空腹血糖 (FBS)及一氧化氮 (NO)等 ,应用聚合酶链反应 (PCR)和限制性片断长度多态性 (RFLP)检测eNOS基因Glu2 98Asp多态性。结果 脑梗死和对照组eNOS基因Glu2 98Asp多态性构成有显著性差异 (χ2 =4 31,P =0 0 38) ,脑梗死组Glu/Asp基因型高于对照组(32 5 %vs 17 8% ) ;脑梗死组 2 98Asp等位基因频率高于对照组 (16 2 5 %vs 8 9% ) ,但是两组等位基因频率的分布比较 ,没有显著性差异 (χ2 =3 81,P =0 0 5 1)。结论 eNOS基因Glu2 98Asp多态性在中国老年人群中存在 ,并且基因型与脑梗死之间存在一定的关系 ;eNOS基因Glu2 98Asp变量可能是中国老年人脑梗死的一种遗传易感指标。

Objective This paper explores the association of Glu298Asp polymorphism of the eNOS gene with cerebral infarction in aged people on the basis of a population, based on case-control study. Methods 40 cerebral infarction random chosen form outpatients and inpatients as study group, and 169 of sex, age-matched health as control subjects. Their height, weight and blood pressure were examined and their fasting plasma lipid concentrations were measured. Glu298Asp polymorphisms of the eNOS gene were measured u...