MBR系统内丝状菌污泥膨胀的分子生态学解析

Molecule Ecology Analysis of Filamentous Bacterium Sludge Bulking in MBR

膜 生物反应器 (MembraneBioreactor ,MBR)工艺是目前污水处理技术的研究的热点之一 .以前大部分研究集中于宏观工程参数控制和活性污泥特性对系统运行的作用过程 ,针对系统内活性污泥的分子生态学研究尚少 .本文在长期试验运行基础上 ,发现丝状菌在MBR运行过程中容易大量增殖形成污泥膨胀 ,对系统的处理效率及膜污染造成一定影响 .为了对其膨胀发生过程及变化进行深入的机理解析 ,本研究利用荧光原位杂交 (FluorescentinSituHybridization ,FISH)分子生物学分析技术 ,对MBR系统内丝状菌污泥膨胀的发生过程进行了跟踪研究 .发现引起膨胀的丝状菌主要是 0 2 1NⅡ型丝状细菌 ,G2M探针杂交后的丝状菌相对量的变化逐渐升高 .

Membrane bioreactor (MBR) is a hotspot technics in wastewater treatment technologies. Now the study of MBR focuses on macro parameter control and system running effected by sludge characteristics, and there are little studies on molecule ecology of active sludge. Sludge bulking frequently in experiment and long-term practice and its negative effect on system operation was discovered in the experiment. In order to know the bulking process, fluorescent in situ hybridization technique to work on the sludge in ...