抗硬化复方对硬皮病小鼠模型影响的研究

Study on Effect of Antisclerosis Composite Prescription on Scleroderma

目的探讨抗硬化复方与青霉胺对硬皮病小鼠模型的影响。方法 C_3H 雌性小鼠52只,皮下注射博莱霉素造模分组给药。以 HE、Masson 染色法观察硬皮病小鼠皮肤厚度及真皮纤维化程度,测定其皮肤羟脯氨酸含量进行皮肤胶原分析,对各组的测定值进行分析比较。结果与模型组比较,中药组、西药组和中西药结合组小鼠皮肤厚度明显变薄(P<0.01),胶原含量有不同程度减少,尤其中西药结合组显著减少(P<0.01)。早予中药组不仅皮肤厚度、胶原含量显著低于模型组(P<0.01),且较 PBS 对照组无明显差异(P>0.05)。结论抗硬化复方药物能明显抑制硬皮病小鼠皮肤胶原纤维增生,减少胶原等细胞外基质的沉积,越早应用,效果越好,中西药联合应用效果更佳。

Objective To explore the effect of Antisclerosis Composite Prescription(ACP)and penicillamine on scleroderma mouse.Methods Fifty-two C_3H female mice were modeled and medicated according to hypodermic injection of bleomycin.The thickness of the skin and fibrosis degree of derma of the sclerotic skin mouse model were observed by HE and Masson staining method to determine the content of hydroxaminoprolin and make an analysis of collagen.Then analytic comparison of the determined values of each group was done....