中西医结合治疗妊娠高血压综合征大鼠的疗效及肾脏内皮型一氧化氮合酶、血管内皮生长因子及FAS表达的变化

Effect of Integrative Chinese and Western Medicine on eNOS,VEGF and FAS Expression in Rats with Pregnancy Induced Hypertension Syndrome

目的探讨川芎嗪合并硫酸镁治疗妊娠高血压综合征(妊高征)大鼠的疗效和机制。方法用亚硝基左旋精氨酸甲酯(L-NAME)尾静脉注射建立大鼠妊高征模型,采用川芎嗪合并硫酸镁(川加硫)治疗,连续3天。通过大鼠套尾法血压测量、24h 尿蛋白测定,观察正常妊娠孕鼠、妊高征孕鼠及分组治疗后的各组孕鼠血压、尿蛋白,新生鼠身长、体重和胎盘重量,结合免疫组化技术检测肾脏内皮型一氧化氮合酶(eNOS)、血管内皮生长因子(VEGF)、FAS 等表达。结果妊娠晚期给予 L-NAME 导致孕鼠血压升高,尿蛋白增加,幼鼠死胎及畸形发生率增加;单用硫酸镁、川芎嗪或两药合用后孕鼠血压、尿蛋白均显著降低(均 P<0.01),而川加硫组较另两组降低更显著(P<0.01);川芎嗪及川加硫治疗后能增加新生鼠身长、提高胎仔体重及胎盘重量(P<0.01);且后者尚能减少胎仔后肢短缩畸形率(P<0.001)。孕鼠给予 L-NAME 后,肾脏 eNOs、VEGF 明显减少,FAS 明显增加。各组治疗后,FAS 着色强度无改变,川芎嗪组、川加硫治疗组 eNOS、VEGF 明显升高。结论 L-NAME 成功建立大鼠妊高征模型;川加硫治疗效果优于单用硫酸镁或川芎嗪。妊高征大鼠肾组织凋亡增加。川芎嗪、川加硫治疗后能降低血压、改善肾功能。

Objective To evaluate the effect and mechanism of ligustrazine(Lz)and magnesium sulfate(MS)in treating preg- nancy induced hypertension syndrome(PIH).Methods Rats model of PIH was established by administration of L- NAME via caudal vein and treated with Lz and MS,singly or in combination(L&M)for 3 days.Blood pressure of rats in different groups(normal,model treated or untreated)were measured by the tail-cuff method and their urinary protein in 24 hrs were also measured.Expression of renal endothelial nitrog...