肾衰胶囊对慢性肾衰竭大鼠肾组织TGF-β1表达的影响

Effect of Shenshuai Capsule on Transforming Growth Factor-β_1 Expression in Kidney Tissue of Rats with Chronic Renal Failure

目的探讨肾衰胶囊对慢性肾衰竭(CRF)大鼠肾脏转化生长因子(TGF-β1)表达的影响。方法大鼠灌胃腺嘌呤制成 CRF 模型,并分为5组肾衰胶囊高、低剂量治疗组及尿毒清治疗组分别以肾衰胶囊4.4g·kg^(-1)·d^(-1)、1.47 g·kg^(-1)·d^(-1)及尿毒清2.0 g·kg^(-1)·d^(-1)灌胃,正常及模型对照组予等体积生理盐水共治疗31天,检测肾功能指标,用免疫组化法检测肾脏 TCF-β_1 表达水平。结果肾衰胶囊高剂量组改善 BUN 、SCr 方面与尿毒清组比较,差异均有显著性(P<0.05)。肾衰胶囊高、低剂量组对 CRF 大鼠肾小管上皮细胞 TGF-β_1表达有明显抑制作用(P<0.01,P<0.05)。结论肾衰胶囊能明显抑制 CRF 大鼠肾组织 TGF-β_1 表达,这可能是其延缓肾衰竭进展的作用机理之一。

Objective To study the effect of Shenshuai Capsule(SSC)on transforming growth factor-β_1(TGF β_1)expression in kidney tissue of rats with chronic renal failure(CRF).Methods Rats were made into the CRF model by gastrogavage of adenine and treated with high dose 4.4 g·kg^(-1)·d^(-1)SSC,low dose 1.47g·kg^(-1)·d^(-1)SSC and Niaoduqing(NDQ,2.0g· kg^(-1)·d^(-1))respectively for 31 days.Criteria of renal functions were measured,and level of TGF-β1 expression was deter- mined by immunohistochemical method.The data ...