都梁丸提取液的镇痛作用及其对小鼠脑组织中5-HT、5-HIAA、DA及NE含量的影响

Analgesic Effect of Duliang Pill Extract and Its Effects on Neurotransmitters 5-HT,5-HIAA,DA,NE Contents in Brain Tissue

目的观察都梁丸提取液的镇痛作用及其对脑组织中5-羟胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、多巴胺(DA)、去甲肾上腺素(NE)含量的影响。方法实验设正常组、模型组、都梁丸提取液大、中、小剂量组;除生理盐水组外,其余各组足趾部注射完全福氏佐剂(FCA)复制炎性疼痛模型后,连续灌胃给药3天,正常组及模型组灌服生理盐水(简称 NS),都梁丸提取液大、中、小剂量组分别灌服都梁丸提取液13.36、6.68、3.34g/kg。热刺激法测定小鼠疼痛模型的痛阈值,高效液相色谱法测定脑组织中5-HT、5-HIAA、DA、NF 含量。结果都梁丸提取液明显提高动物模型痛阈,增加脑组织中5-HT、5-HIAA、DA 含量,降低 NE 的含量,与模型组比较有差异显著性(P<0.05)。结论都梁丸提取液具有镇痛作用,其机制可能与其影响痛觉调制的神经递质5-HT、5-HIAA、DA、NE 含量有关。

Objective To observe the analgisic effect of DLP extract and its effects on neurotransmitters 5-HT,5-HIAA,DA and NE in brain tissue.Methods Mice were randomly assigned to normal,model and DLP high,medium and low con- centration group,which were treated with NS and DLP extract 13.36 g/kg、6.68 g/kg、3.34 g/kg respectively for three days.Pain threshold was measured by heat stimulation method.Contents of 5-HT,5-HIAA,DA and NE in brain tissue were assayed by high performance liquid chromatography (HPLC).Results D...