电针对脊髓损伤大鼠早期bax mRNA及蛋白表达的影响

Effect of Electron Acupuncture on bax mRNA and Protein Expression in Rats with Early Stage Spinal Cord Injury

目的探讨电针对脊髓损伤后 bax mRNA 及蛋白表达的影响。方法成年雄性 SD 大鼠,随机分为模型组、电针治疗组、甲基强的松龙治疗组及假手术组。采用改良的 Allen’s 锤击法致大鼠 T_(10)脊髓损伤,应用原位杂交和免疫组织化学方法及图像定量分析法观察脊髓损伤早期 bax mRNA 和蛋白的表达。结果假手术组有中等量的 bax mRNA 及蛋白表达。模型组脊髓损伤后6h 及24h bax mRNA 及蛋白表达均增高,电针治疗组 bax mRNA 及蛋白表达均低于模型组,与甲基强的松龙组比较差异无显著性。结论电针可下调bax mRNA 及蛋白的表达,从而抑制细胞凋亡,保护神经细胞。

Objective To study the effect of electron acupuncture (EA) on bax mRNA and protein expression after spinal cord injury.Methods Adult male SD rats were randomly divided into the model group,the EA group,the methylprednisolone (MP) group and the sham operated group.Spinal cord injury of T_(10) in rats were caused by modified Mllen s hammering method.Results The bax mRNA and protein of moderate quantity were found in the sham operated group,the parame- ters increased in 6 hrs and 24 hrs after spinal cord injur...