抗新疆出血热病毒单克隆抗体识别病毒蛋白质的实验观察

Experimental Observation on Recognition of Viral Protein by Monoclonal Antibodies to XHF Virus

对本实验室建立的6株杂交瘤细胞株分泌的单克隆抗体识别新疆出血热病毒蛋白质的性质进行了实验观察。用新疆出血热病毒原型毒株BA66019感染乳鼠脑的20%悬液,经差速离心后的上清为抗原进行SDS-PAGE电泳,再用Westernbolt法将凝胶分离的结果印迹到NC膜上。将6株单克隆抗体小鼠腹水经饱和硫酸铵纯化后,在NC膜上进行结合反应。用直接和间接免疫酶染色法显示结合位点。同时以新疆出血热恢复期病人血清、抗正常鼠脑免疫腹水和抗人IgG血清做对照。实验结果证明这6株杂交瘤分泌的单克隆抗体都是针对病毒核衣壳蛋白(N多肽)的抗体。

The experiments were carried out for definition of viral protein recognition of 6 monoclones secreting antibodies to XHF virus. The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique .The result demonstrates all monoclonal antibodies directed against viral nucleocapsid protein of Xinjiang hemorrhagic fever virus.