正交试验优选金银花中总黄酮的最佳提取工艺

Study on Extracition process of flavonoids In Flos by the method of orthogonal test optimization

目的:探索金银花中总黄酮最佳提取工艺。方法:采用正交法考察乙醇浸泡浓度,萃取时间、乙醇洗脱浓度,物料比 4 个因素对金银花中总黄酮得率的影响。结果:金银花中总黄酮的最佳提取工艺;用 30 倍于样品重的 80%的乙醇浸泡 24h 后继用超声法提取 40min,以 60%的乙醇洗脱。结论 以超声波法提取的总黄酮含量高,操作简便,节省燃料和时间,提取较完全,此法宜工业化生产。

OBJE CTIVE:study on extro ction process of flovonoids in Flos Lonicerae. METHODS:The orthogonal test was adopted to ve search the effects of 4 factors on the yielding rate of flavo noids from flos lonicerae, RESULTS:The concentration of 80% ethanol was 30 times raw herbtodouse the dried the dried flos Lonicevae for 24 hours,and altrasonic treatment lasted for ane hour.The extraction of flavonoids was as high as 3.2%. CONLUSION:This process is characterized with simple operation safe operate s...