诱导型一氧化氮合酶在单侧输尿管梗阻大鼠肾脏的表达

The renal expression of inducible nitric oxide synthase in rats with unilateral ureteral obstruction

目的 :探讨诱导型一氧化氮合酶 (iNOS)在单侧输尿管梗阻 (UUO)大鼠术侧肾脏的表达。方法 :建立左侧输尿管梗阻模型 (UUO组 ) ,设假手术组为对照。 3d后应用逆转录 聚合酶链反应 (RT PCR)检测iNOS的mRNA水平。结果 :与对照组相比 ,UUO组大鼠肾脏出现明显病理变化 ,并且其iNOSmRNA表达明显增加。结论

Purpose:To observe the renal expression of inducible nitric oxide synthase(iNOS)in rats with unilateral ureteral obstruction(UUO). Methods:Rats were randomly divided into two groups.Some were subjected to complete ligation of left ureter and the others with sham operation were used as control. Three days later the mRNA expression of iNOS in left kidney of all these rats were examined by reverse transcription polymerase chain reaction (RT PCR) method.Results:The gene expression of iNOS in the UUO group was much higher than those in the control group.Conclusions:iNOS was involved in the pathogenesis of the injuries of UUO.