965个体外受精-胚胎移植周期的临床应用

Clinical Outcome of 965 cycles of In Vitro Fertilization and Embryo Transfer

目的 总结本中心2001年1月1日-2002年9月30日两年来开展体外受精-胚胎移植(IVF-ET)治疗不孕症的临床情况。方法 使用GnRH-a/FSH/HMG/HCG超促排卵方案行常规IVF或ICSI及冻融胚胎移植。分析比较2001年于2002年两阶段的临床结果。结果 2001年进行490个开始周期,完成移植周期423个,临床妊娠率为28.13％,受精率为63.17％;2002年进行475个开始周期,完成移植周期382个,临床妊娠率为36.91％,受精率为76.17％。2002年临床妊娠率及受精率均较2001年有明显的提高,分别为P<0.01、P<0.001。2001年进行69个周期冻融胚胎移植,19个周期临床妊娠,临床妊娠率为27.5％,2002年冻融胚胎移植208个周期,65个周期临床妊娠,临床妊娠率为31.3％。结论 实验室人员操作技术、胚胎移植技术的提高及实验室环境逐步稳定是提高成功率的主要因素。

Objective To review 965 cycles of in vitro fertilization and embryo transfer (IVF-ET) programs from January 1,2001 and September 31,2002 in our center. Methods a total of 965 cycles including conventional IVF and intracytoplasmic sperm injection (ICSI) therapy. The clinical outcome were compared in the year of 2001 and 2002 respectively. Results During the year of 2001, there were 490 treatment cycles, 423 were transferred, and there were 475 treatment cycles, 382 were transferred in 2002. The clinical pregnancy rate and inseminated rate were 36.91% an 76.17% respectively, statistically significantly higher in the year of 2002 than that in the year of 2001, which were 28. 13% and 63.17% (P< 0. 01, P< 0. 001). Also, we had 69 cycles of frozen thawed embryos transfer, the pregnancy rate was 27. 5%(19/69) in the year of 2001, while, 208 cycles, rate, 31. 3%(65/208) in the year of 2002. Conclusions All the IVF procedures do affect the quality of embryos. The improvement of IVF technique, and the factor in laboratory are the key factors leading to a successful clinical outcome.