期刊文献+

The study on three-dimensional reconstruction of endometrium in vitro

The study on three-dimensional reconstruction of endometrium in vitro
下载PDF
导出
摘要 Objectives:To establish a three-dimensional model of endometrium in vitro.Methods: The endometrial cells were cultured on a biodegradable hybrid Collagen-PGLA polymer mesh and observed by scanning electron microscopy and light micro-scopy.Results:It showed that the endometrial cells adhered and spread well on the surfacesof the collagen sponge of the hybrid mesh after being cultured for 5 days. After 2weeks, the cells proliferated to become confluence achieving a three dimensional struc-ture.Conclusion: The three-dimensional model of endometrium could be establishedusing the hybrid polymer mesh as a skeleton in vitro. Objectives:To establish a three-dimensional model of endometrium in vitro. Methods:The endometrial cells were cultured on a biodegradable hybrid Collagen- PGLA polymer mesh and observed by scanning electron microscopy and light micro- scopy. Results:It showed that the endometrial cells adhered and spread well on the surfaces of the collagen sponge of the hybrid mesh after being cultured for 5 days.After 2 weeks,the cells proliferated to become confluence achieving a three dimensional struc- ture. Conclusion:The three-dimensional model of endometrium could be established using the hybrid polymer mesh as a skeleton in vitro,
出处 《生殖医学杂志》 CAS 2003年第z1期19-23,共5页 Journal of Reproductive Medicine
关键词 COLLAGEN BIODEGRADABLE Poly DL-lactic-co-glycolic acid Endome-trial cell TISSUE engineering Collagen Biodegradable Poly DL-lactic-co-glycolic acid Endome-trial cell Tissue engineering
  • 相关文献

参考文献14

  • 1[1]Langer R, Vacanti JP. Tissue engineering. Science,1993, 260(5110): 920-926.
  • 2[2]Mooney DJ, Mikos AG. Growing new organs. Sci Am, 1999, 280(4): 60-65.
  • 3[3]Chen GP, Ushida T, Tateishi T. A biodegradable hybrid sponge nested with collagen microsponges. J Biomed Mater Res, 2000, 51(2):273-279.
  • 4[4]Chen GP, Ushida T, Tateishi T. Poly(DL-lactic-coglycolic acid) sponge hybridized with collagen microsponges and deposited apatite particulates. J Biomed Mater Res,2001,57(1) :8-14.
  • 5[5]McCormack SA, Glasser SR. Differential response of individual uterine cell types from immature rats treated with estradiol. Endocrinology, 1980, 106 (5):1634-1649.
  • 6[6]Peter SJ, Miller MJ, Yaszemski MJ, et al. Polymer concepts in tissue engineering. J Biomed Mater Res,1998, 43(4) :422-427.
  • 7[7]Freed LE, Vunjak-Novakovic G, Biron RJ,et al. Biodegradabal polymer scaffolds for tissue engineering.Bio/Technology, 1994,12(7) :686-693.
  • 8[8]Mikos AG, Sarakinos G, Leite SM, et al. Laminated three-dimensional biodegradable forms for use in tissue engineering. Biomaterials, 1993,14(5): 323-330.
  • 9[9]Kim BS, Mooney DJ. Development of biocompatible synthetic extracellular matrices for tissue engineering.TIBTECH, 1998,16(5) :224-230.
  • 10[10]Hansbrough JH, Morgan JL, Greenleaf GE, et al.Composite grafts of human keratinocytes grown on a polyglactin mesh-cultured fibroblast dermal substitute function as a bilayer skin replacement in full-thickness wounds on athymic mice. J Burn Care Rehabil,1993,4(5) :485-494.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部