黑麦草组织的快速繁殖

Tissue Culture of Lolium Perenne for Rapid Propagation

为加快草坪草无性系的选择、推广和应用,以黑麦草成熟种子为外植体进行组织培养,筛选出较理想的愈伤组织分化、芽分化、增殖和生根培养基,即愈伤组织培养基选用MS+2.0 mg/L2,4-D+0.5 mg/L6-BA,愈伤率可达80.0%;芽分化、增殖培养基为MS+0.1 mg/L 2,4-D+1.0mg/L6-BA,芽分化率可达44.8%;生根培养基为1/2MS+0.5 mg/LNAA,生根时间短,生根率可达到88.2%以上.