鹿茸生长素对维甲酸所致大鼠骨质疏松影响的实验研究

Experimental researches on the influence of hairy deerhorn auxin on the rat's osteoporosis induced by the retinoic acid

目的 探讨鹿茸生长素对维甲酸所致大鼠骨质疏松的疗效作用机制。方法 Wistar大鼠60只,随机分为正常对照组、模量组、密钙息组及鹿茸生长素高、中、低剂量组各10只,用维甲酸诱导大鼠骨质疏松模型为对象,测24 h尿中总尿钙、磷、肌酐和羟脯氨酸和血浆中磷、碱性磷酸酶。并测定股骨的骨密度、骨重、骨长、骨直径、抗弯强度和骨钙含量,并对结果进行统计分析。结果 经鹿茸生长素治疗后,维甲酸所致骨质疏松大鼠骨密度、骨重、骨长均有不同程度的升高,抗弯强度和骨钙含量也明显升高,与密钙息组比较差异无显著(P<0.01),有一定量效关系,但对骨直径影响不明显。高剂量治疗组和药物对照组均能明显提高维甲酸所致骨质疏松大鼠组织的相对骨体积和平均骨小梁宽度,二者之间差异无显著性(P<0.01)。结论 鹿茸生长素对维甲酸所致大鼠骨质疏有明显治疗作用。

Objective To investigate the mechnism of the therapeutic effectiveness of hairy deerhorn on the rat's osteoporosis, induced by retinoic acid. Methods Sixty Wistar rats were divided randomly into six groups,namely normal control,model group,Miacalcic group and hairy deerhorn auxin group with high,medium, and low dose, 10 cases for each group. The rats osteoporosis was induced with retinoic acid, the amount of Ca,P,Creatinine,hydroxyproline,in urine, and phosphorum,and phosphatase in plasm of the rats' collected, during the twenty-four hours, were be measured,and the density, weight,length,diameter, intensity of ani-cur-vature and the quantity of the Ca of the femur also were be surveved and the statistical analysis on the results achieved were be made. Results After the application of hairy deerhorn auxin to treat the osteoporosis of rats, induced by retinoic acid,the density,weight,length of their bone have been risen at different levels,just as the same as the intensity of the anti-curvature of that of miacalcic groups. They are no significant difference with that of Miacalci group(P>0.01). There were only some effective relationships,but their effection on diameter of the bone is not significant. High-dose treating group and medicinal controlled group can both rise the relative bone volume of the ill rat's bone tissue and the average width of the bone trabeculae. There are not significant differences between them(P>0.01).Conclusion The hairy deerhorn auxin can cure the rafs osteoporosis, induced by the retinoic acid effectively.