高渗盐水治疗脊髓急性压迫损伤的实验研究

Effect of hypertonic saline on acute spinal cord compression in rats

目的 观察高渗盐水对脊髓损伤的治疗作用,并探讨其作用机制。方法 36只SD大鼠造成T10节段脊髓急性压迫损伤后,随机分为三组:高渗盐水治疗组、生理盐水治疗组及对照组,每组观察损伤后1、4周两时间段;评价动物神经功能,观察各时间段脊髓病理改变,计算脊髓残留组织保留率。结果 ①高渗盐水治疗组动物神经功能恢复更快、更完全,与生理盐水组及对照组相比差异有显著意义(P<0.05);②损伤后1周,高渗盐水治疗组脊髓组织炎症反应、水肿明显减轻;③损伤后4周,高渗盐水治疗组脊髓残留组织保留率显著增加,与其它两组相比差异有显著性意义(P<0.01)。结论 高渗盐水能减轻脊髓组织病理改变,促进神经功能恢复;减轻损伤后脊髓组织的炎症反应及水肿是其作用机制之一。

Objective To observe the effect of hypertonic saline on acute spinal cord compression and investigate its mechanisms Methods 36 SD rats were randomized into three groups after experienced acute spinal cord compression at the level of T10:The hypertonic saline treated group,normal saline treated group and control group. The observing period in each group was as following: 1 week post injury and 4 week post injury. All animals were sacrificed after evaluating the neurological function at the end of the observing period. T9-11 spinal cords were removed after perfusion fixation. We observed the pathological changes of spinal cord at each observing period and measured the spared tissue area of spinal cord at 1st and 4th weeks post injury. Results There was significant better neurological recovery in the hypertonic saline treated group than in the others (P <0.05).The edema and inflammation of spinal cord in the hypertonic saline treated group was lighter than that in the other two at 1 week post injury. Accordingly, the extent of spared tissue of spinal cord increased significantly in the hypertonic saline treated group at 4 week post injury (P<0.01). Conclusion These results strongly suggest that the hypertonic saline can reduce the pathological changes of spinal cord injury, and improve the recovery of nervous function. Alleviating edema and inhibiting inflammation of spinal cord is one of the mechanisms of this effect.