磷酸钙骨水泥人工骨的制备及修复兔桡骨大段骨缺损的实验研究

Preparation of calcium phosphate cement as artificial bone and experimental studies on the repair of rabbits'segmental defects of radius

目的 制备水泥型羟基磷灰石(CPC)人工骨,并研究CPC人工骨修复兔桡骨大段骨缺损的成骨效果。方法 高温法制备出磷酸四钙,然后在模拟体内环境下将其与无水磷酸氢钙发生水化固化反应,合成水泥型羟基磷灰石人工骨,将自制的CPC人工骨植入新西兰兔桡骨大段骨缺损模型中,术后4、8、12、16周取材,采用HE染色和Masson三色染色分析评价骨缺损修复效果。结果 CPC植入后4周有新生软骨形成及未成熟的骨组织,可见大量的软骨细胞、成骨细胞、胶原组织及较多的小血管。8～16周新骨继续生成,人工骨逐渐改建为成熟的板层骨、骨小梁和髓腔结构。结论 CPC人工骨制备简单,有良好的生物相容性和成骨效果,可能是一种较理想的骨移植替代材料。

Objective To prepare the tetra-calcium phosphate cement(CPC)in higher temperature and study it' s effect in the repair of rabbits' segmental defects of radius. Methods Tetra-calcium phosphate (TTCP) was prepared by high heating, than imitated the internal situation of humanbody, for hydrolization and solidation,to synthesize the hydroxy-calcium phosphate,as artificial bone in sement-type(CPC) ,then im-planteted them into the large segmental defect of radius of New Silan rabbits model. The implanted materials were taken out at 4th, 8th, 12th, and 16th weeks after implantation. The histological sections, with HE and Masson's trichromic staining made, to evaluate the effectiveness of repair of bony defect. Results There existed new cartilage and new immature bone tissue in the bone substitute in 4 weeks after operation, as well as mass of cartilageous cells,osteoblasts, collagen tissue and more small vessels were seen. In the period of 8 to 16 weeks after operation,new bone was formed continuously, artificial bone gradually reconstructed to mature lamellar bone,bone trabeculae and medullary cavity,etc. Conclusion The preparation of CPC is simple. Its effectiveness of biocompatibility and osteogenesis is excellent and it may be an ideal bone substitution. Its clinical application in future will be very hopeful.