丹参与骨碎补注射液防治激素诱发股骨头坏死的实验研究

Experimental research on prevention of steroid-induced avascular necrosis of femoral head with injection of Salvia Miltiorrhiza and Drynaria

目的 探讨丹参和骨碎补对激素性股骨头坏死的防治机理及其交互作用。方法 选用家兔50只,随机分成五组:模型组、丹参组、骨碎补组、丹参+骨碎补组、对照组。8周后观察五组家兔血液流变学、脂代谢、股骨头组织学等指标的改变。结果 与对照组相比,模型组股骨头疏松,易于凿切,血液粘度和血脂升高,骨小梁变细、稀疏,空骨陷窝率增高,髓腔脂肪细胞增多;而其余各组上述病变有不同程度的减轻,其中以丹参+骨碎补组病理改变最轻。结论 丹参、骨碎补均能预防激素所致的高粘滞血症和高脂血症;骨碎补能提高血钙血磷,激活成骨细胞,提高股骨头的骨密度,能预防激素性骨质疏松;两药在预防SANFH方面有协同作用。

Objective To investigate and compare the protective effects of Salvia Miltiorrhiza and Dry-naria on steroid-induced avascular necrosis of femoral head(SANFH). Methods 50 adult rabbits were randomly divided into five groups: Model group (Group A, n = 10), Salvia Miltiorrhiza group (Group B, n = 10), Dryraria group (Group C,n = 10),combined use of Salvia miltorrhiza and Drynaria group (Group D, n = 10) and control group(Group E, n = 10). Indexes of slice pathomorphology of bone, hemorheology, serum calcium and phosphor,fatty metabolism and bone mineral density (BMD) were performed eight weeks later. Results Compared with Group E,Group A showed significant changes:such as osteoporosis of femoral head which was easier to be cut,the blood and plasma viscosity and lipid raised;the presence of more bone lacuna,thinner tra-beculae and lower bone mineral density (BMD) and more fat cells in the medullary cavity. The pathological changes above mentioned in Group B,C and D were lightened in different degree. The blood and plasma viscosity in Group B and C were decreased. The serum calcium and BMD of femoral head increased in Group C and D. Conclusion Either Salvia Miltiorrhiza or Drynaria could prevent hemorheological condition and lipid metabolism;Drynaria is able to raise serum calcium and BMD of femoral head,activate osteoblasts, elevate os-teo-density,and to prevent from osteoporosis; Sal via Miltiorrhiza pocesses a synergetic effect with Drynaria in preventing from SANFH.