细菌感染大鼠血中血小板型磷脂酶A_2 mRNA水平的变化及其cDNA克隆

Platelet phospholipase A_2 mRNA content changes and cDNA cloning in rat blood with bacteria infection

目的 观察感染大鼠血中血小板型PLA2 mRNA水平的变化 ,研究其基因和氨基酸顺序结构的特征。为开发新的抗菌药物提供依据。方法 用半定量RT PCR方法检测感染大鼠血中PLA2 mRNA ,并对PLA2 cDNA进行克隆和序列分析。结果 大鼠感染细菌后 ,血中血小板型PLA2 mRNA水平迅速明显增加 ,其DNA和氨基酸结构与其他组织来源的PLA2 有高度的同源性。结论 细菌感染可引起大鼠血中PLA2 在基因水平上的迅速反应 ,这可使血小板型PLA2 的生成增加并控制感染。血中克隆出来的PLA2 cDNA与大鼠其他组织来源的PLA2 虽略有不同 。

Aim To explore the changes of rat platelet phospholipase A 2 (PLA 2) mRNA content in bacteria infected rat and study the cDNA and amino acid sequences of the PLA 2 structure to lay a good foundation for the development of new antibiotics. Methods The PLA 2 mRNA level in blood was determined by RT PCR. The DNA sequence was cloned and analyzed. Results After injection of bacteria in rats, the mRNA level of PLA 2 in blood increased markedly. The cDNA and amino acid sequence were highly homologous to o...