RP-HPLC法测定大鼠血浆中丹参酮IIA浓度及其药代动力学研究

Determination of tanshinone IIA in rat plasma and the pharmacokinetics by RP-HPLC method

目的 建立高效液相色谱法测定大鼠血浆中丹参酮IIA浓度的方法。方法 血浆样品经液 液萃取后 ,用HPLC法进行分析。色谱柱为YMCC1 8(5 μm ,ID 3 0mm× 1 50mm) ;流动相为乙腈 水 冰醋酸 (74∶2 6∶1 ) ;流速 0 3mL·min-1 ;检测波长 2 70nm ;内标为 4 氯联苯。结果 线性范围为 0 0 5mg·L-1 ～ 6 40mg·L-1 ,最低检测浓度为 0 0 5mg·L-1 。高、中、低 3种浓度的平均方法回收率分别为 98 9% ,1 0 2 1 %和 1 0 0 4%。日内、日间精密度 (RSD)均小于5 %。结论 本方法稳定、简便、可靠 。

Aim To develop a sensitive and rapid HPLC method for the determination of tanshinone IIA (TS) in rat plasma and to study its pharmacokinetics in rats. Methods TS and 4 chlorodiphenyl (internal standard) were extracted from plasma with ethyl acetate. After liquid liquid extraction, the sample was analyzed by HPLC with YMC C 18 column (5 μm, 150 mm×3 0 mm ID). The mobile phase consisted of acetontrile water acetic acid (74∶26∶1) at the flow rate of 0 3 mL·min -1 , the UV detection wave length...