寡核苷酸的摄取与血液肿瘤细胞种类和增殖活性的关系

Oligonucleotide uptake in hematological tumor cells is related to cellular species and proliferation

目的 探讨血液肿瘤细胞摄取寡核苷酸与其种类和活性的关系。方法 流式细胞仪测定细胞内的平均荧光强度。结果 经过 0 60 μmol·L-1 荧光素FITC标记的G31 39作用 4h后 ,血液系统肿瘤患者外周血及骨髓单个核细胞对G31 39的摄取能力明显高于正常人 ;不同来源的血液肿瘤细胞株摄取G31 39的能力不同 ,单核细胞、B淋巴细胞和髓系粒细胞来源的白血病细胞的摄取能力明显高于T淋巴细胞来源的白血病细胞 ;经过全反式维甲酸作用的HL60细胞的增殖活性明显受到抑制 ,同时细胞摄取G31 39的能力明显下降。结论 血液肿瘤细胞具有摄取寡核苷酸的能力 。

Aim To explore whether the oligonucleotide upkake in hematological tumor cells is related to cellular species and proliferation. Methods Intracellular mean fluorescence intensity was measured by flow cytometry. Results After treatment with FITC-labeled G3139 at the concentration of 0 60 μmol·L -1 for 4 h, the G3139 uptake into peripheral blood mononuclear cell and bone marrow mononuclear cell in hematological tumor patients was significantly higher than that in normal control. There was different u...