大鼠一次性灌服酸枣仁提取物后棘苷的药代动力学研究(英文)

Pharmacokinetic study on spinosin in rat plasma after oral administration of Suanzaoren extract at a single dose

目的 用反相高效液相色谱法 ,以磺胺甲唑为内标 ,对一次性ig酸枣仁提取物后的大鼠血浆中棘苷进行药代动力学研究。方法 血浆样品经乙腈沉淀蛋白后 ,于 50℃氮气流下吹干 ,残渣用流动相溶解后进行分析。色谱条件为色谱柱 :HypersilC1 8柱 ,2 0 0mm× 4 6mmID ,5μm ;流动相 :乙腈 水 冰醋酸 (1 5∶85∶1 ) ;流速 :0 7mL·min-1 ;检测波长 :334nm ;柱温 :35℃。结果 血浆中棘苷在 1 8 1～ 90 3 5μg·L-1 成良好线性关系 (r2 ≥ 0 995)。平均回收率为94 5 % ,日内、日间精密度RSD均小于 9 0 %。该法定量限为 1 8 1 μg·L-1 ,血浆样品在 - 2 0℃可稳定保存。结论 该法简便、灵敏、准确 。

Aim To study the pharmacokinetics of spinosin in rat plasma after oral administration of Suanzaoren extract using sulfamethoxazole (SMZ) as internal standard by RP-HPLC method. Methods Plasma samples were deproteined with acetonitrile, followed by evaporation of the acetonitrile to dryness. The residual was then resolved in mobile phase and HPLC separation was achieved on a Hypersil C 18 (5 μm, 200 mm×4 6 mm ID) column at 35 ℃. The mobile phase consisted of acetonitrile-water-acetic acid (15∶85∶1) a...