心血管相关基因芯片的制备及其在知母皂苷作用机理研究中的应用

Investigations on the molecular mechanisms of saponins from Anemarrhena asphodeloides Bunge using oligonucleotide microarrays

目的 研究知母皂苷活血化瘀作用的分子机制。方法 制备了包含 87个心血管疾病相关基因探针的寡核苷酸芯片。将 80mg·L- 1 知母皂苷作用 8h及未作用的培养人血管内皮细胞提取总RNA ,通过逆转录分别标记Cy 5和Cy 3荧光染料 ,然后与芯片杂交 ,检测知母皂苷对血管内皮细胞中心血管疾病相关基因表达的影响。 结果知母皂苷作用于血管内皮细胞后 ,血管紧张素酶原 (AGT)基因、肾上腺素α2A受体 (ADRA2R)基因和内皮素转换酶 1(ECE 1)基因的表达均有不同程度的下调。结论 知母皂苷有调控血管内皮细胞功能的作用 ,可能是其治疗心血管疾病的机制之一。

Aim To investigate the molecular mechanisms of saponins from the rhizome of Anemarrhena asphodeloides Bunge. Methods Oligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed. Effects of saponins on gene expression in human umbilical vein endothelial cells were analyzed by comparing hybridization of Cy 5-labeled cDNAs from saponins-treated human umbilical vein endothelial cells and Cy 3-labeled cDNAs from untreated human umbilical vein ...