脂质体粒径的分光光度法检测(英文)

Spectrophotometry on measuring the size of liposomes

目的 确立分光光度法判断脂质体相对大小 ,为评估脂质体物理稳定性提供一种简便、快捷的方法。方法 以Rayleigh Gans Debye理论为基础 ,固定脂质体膜材的种类、比例及其在溶液中的总量 ,分别用乙醇注入法和高压乳匀法制备不同粒径的空间稳定脂质体 ,以电镜法和热力学光散射法测得的粒径作为标准 ,找出不同方法制得脂质体在 4 36nm波长处的吸光度A与粒径D的关系。结果 单位磷脂浓度的脂质体溶液在 4 36nm处log(A4 36nm Cp)与脂质体粒径的对数logD呈线性相关 (r2 0 93,n =5 )。结论 脂质体溶液的吸光度能反映脂质体相对大小 ,可作为一种评定脂质体物理稳定性的方法。

Aim To establish a spectrophotometric method for measurement of the sizes of liposomes for evaluating physical stability of liposomes. Methods The sterically stabilized liposomes (SLs) were prepared by ethanol injection method and extrusion method. The mean cumulant diameters ( D ) of the vesicles were determined by electron microscopy and dynamic light scattering. On the basis of Rayleigh-Gans-Debye theory, the absorbance at 436 nm per unit lipid concentration ( A _ 436 nm / C _p) was measured as a fun...