摘要
目的 研究caspases在缺氧性脑微血管内皮细胞凋亡中的作用。方法 用氰化钠合并无糖培养基造成培养的牛脑微血管内皮细胞缺氧 ;用台盼蓝染色、TUNEL及流式细胞仪计数方法观察细胞受损和凋亡情况 ;用免疫细胞化学染色法观察受损细胞中caspase 3的表达。结果 氰化钠合并无糖培养基可损伤牛脑微血管内皮细胞 ,使细胞发生凋亡 ,受损细胞中caspase 3大量表达。广谱caspases抑制剂、选择性caspase 1 , 3和 6抑制剂均能显著减少细胞死亡数目、caspase 1和 6抑制剂可以抑制caspase 3的表达。结论 Caspases在缺氧性脑微血管内皮细胞凋亡过程中起重要作用 ,在caspases的蛋白酶级联切割反应中caspase 3位于caspase 1和
Aim To study the effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by hypoxia in vitro . Methods The cultured bovine cerebromicrovascular endothelial cells were exposed to NaCN in glucose free medium. Cell viability was determined by trypan blue staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase mediated dUTP nick endlabeling (TUNEL) and flow cytometry. The expression of caspase 3 was detected by immunocytochemical method. Four caspase inhibito...
出处
《药学学报》
CAS
CSCD
北大核心
2003年第10期739-742,共4页
Acta Pharmaceutica Sinica