胎鼠神经干细胞培养方法的建立及药物对干细胞增殖的影响

Culture of neural stem cells from cerebral cortex of rat embryo and effects of drugs on the proliferation ability of stem cells

目的 建立神经干细胞培养模型 ,观察药物对其增殖能力的影响。方法 建立大鼠胎脑神经干细胞的培养方法 ,并用MTT法和3 H 胸腺嘧啶核苷参入法观察黄皮酰胺、丹酚酸A及人参皂苷Rg1等对第 2代神经球细胞状态的影响。结果 免疫细胞化学结果显示 ,培养的神经细胞具备干细胞的基本特性 ;MTT和液闪结果则表明 ,上述3种药物可不同程度地影响神经干细胞的存活率和 或增殖活性。

Aim To establish culture procedures of neural stem cells from embryonic rat brain, determine their stem cell characteristics and observe the effects of several compounds on their proliferation ability. Methods Firstly, a stem cell culture system was set up from embryonic rat cortex. The cells were identified as neural stem cells through immunocytochemistry, in which antibodies to neural stem cell specific protein and markers of mature neural cells were used. Then, by using MTT assay, the survival rate of...