灯盏乙素在兔体内药代动力学(英文)

Study on pharmacokinetics of scutellarin in rabbits

目的 建立测定灯盏乙素血浆浓度的固相萃取 高效液相色谱 (SPE HPLC)法 ,并研究家兔iv灯盏花素的药代动力学。方法 采用甲醇 水 磷酸为流动相 ,NucleosilC1 8色谱柱为固定相 ,紫外检测波长 335nm ,外标法定量。给家兔分别iv灯盏花素 1 0 ,2 0和 40mg·kg-1 ,SPE HPLC法检测血浆药物浓度。结果 线性范围 0 0 2～ 1 0 0mg·L-1 ,最低检测浓度 0 0 2mg·L-1 ,方法回收率 96 1 5 %～ 99 31 % ,日内、日间RSD值均小于 1 0 %。家兔iv灯盏花素时 ,灯盏乙素血浆浓度变化符合三房室模型。灯盏乙素低、中剂量组的主要药代动力学参数无显著性差异 ,而高剂量组与低、中剂量组比较差异显著。结论 该法准确、灵敏、简便 ,适用于灯盏乙素血浆浓度的测定。灯盏花素经兔iv后的药代动力学符合三房室模型 ,剂量为 1 0～ 2 0mg·kg-1 时 ,药物的体内变化为线性动力学过程 ,而 40mg·kg-1

Aim To establish a solid phase extraction high performance liquid chromatographic (SPE HPLC) method for determining plasma scutellarin concentration, and study its pharmacokinetics after iv breviscapine in rabbits. Methods Methanol water phosphoric acid (50∶50∶0 5) mixture was used as mobile phase, Nucleosil C 18 column (250 mm×4 6 mm ID) was selected. The wavelength of UV detection was 335 nm. Fifteen rabbits, randomized into 3 groups, were given breviscapine iv at the dose of 10, 20 and 40 ...