黄芩苷元选择性诱导人白血病K562细胞凋亡

Baicalein selectively induce apoptosis in human leukemia K562 cells

目的 研究黄芩苷元诱导人白血病细胞凋亡的作用及机理。方法 MTT法测细胞毒活性 ,Hoechst 332 5 8荧光染色法观察凋亡小体形成 ,流式细胞仪AnnexinVFITC PI法检测细胞凋亡发生率 ,PI染色法检测凋亡峰及细胞周期 ,同时流式细胞仪检测细胞Bcl 2 ,Fas和Caspase 3蛋白表达情况。结果 黄芩苷元能选择性抑制人白血病K5 6 2细胞生长且呈浓度依赖关系 ,并能诱导细胞凋亡 ,细胞增殖被阻滞于S期 ;同时细胞Fas和Caspase 3蛋白表达增高 ,而Bcl 2蛋白表达不变。结论 黄芩苷元能激活Caspase 3蛋白表达 ,诱导人白血病K5 6 2细胞凋亡且呈时效量效关系 ,此作用与Fas蛋白表达上调有关 ,与Bcl

Aim To study the antitumor effect of baicalein on human leukemia K562 cell and its mechanism. Methods The IC 50 value and cytotoxity of K562 cell were detected by MTT method. The apoptotic cell was analyzed by FCM using Annexin V FITC - PI staining method. Sub-G 1 peak was also measured by FCM. Protein expressions of Bcl-2,Fas, Caspase 3 were evaluated with FCM. Results Baicalein was shown to significantly inhibit the proliferation of K562 cell in a dose-dependent manner and selectively induce apopto...