吲哚美辛对脂多糖引起的人类风湿性关节炎滑膜细胞中白介素-6表达的影响

Effect of indomethacin on expression of interleukin-6 caused by lipopolysaccharide in rheumatoid arthritic patients’ synoviocyte

目的 研究吲哚美辛对脂多糖引起的人类风湿性关节炎 (RA)成纤维状滑膜细胞 (FLS)中白介素 6 (IL 6 )表达的影响。方法 用放免分析法检测IL 6蛋白的表达水平 ;RT PCR方法测定IL 6mRNA的表达水平。结果LPS处理对FLS中IL 6表达无显著影响 ;LPS刺激的U937细胞培养液上清液可明显增强FLS中IL 6蛋白分泌及mRNA表达 ;吲哚美辛可显著抑制上述变化 ,且其抑制作用随浓度的增加而增强。结论 吲哚美辛可抑制LPS刺激的U937细胞培养上清液引起的FLS中IL

Aim To study the effects of indomethacin on interleukin-6 (IL-6) expression stimulated with lipopolysaccharide (LPS)in rheumatoid arthritic patients synoviocyte. Methods Fibroblast-like cells (FLS) from rheumatoid arthritic patients joint tissue were cultured for 24 h and incubated 24 h with LPS (1 mg·L -1) or the supernatant of U937 cells stimulated by LPS (1 mg·L -1) . After indomethacin or dexamethasone added into the supernatant of U937 cells, FLS was incubated with the super natant for 24...