摘要
Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15∶18∶66∶1) for ES +, acetonitrile-methanol-water (15∶18∶67) for ES -. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L·h -1 . The ion source temperature was 150 ℃. Results The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity. Conclusion It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.
Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4...
出处
《药学学报》
CAS
CSCD
北大核心
2003年第12期968-970,共3页
Acta Pharmaceutica Sinica
关键词
二苯乙烯苷
液-质联用
药物代谢
葡糖醛酸
stilbene glucoside
HPLC-MS
drug metabolite
glucuronide conjugate