7-羰基-脱氢表雄酮对慢性轻度应激BALB/c小鼠免疫系统的影响

Effects of 7-oxo-DHEA treatment on the immunoreactivity of BALB/c mice subjected to chronic mild stress

目的 建立慢性轻度应激动物模型 ,观察 7 羰基 脱氢表雄酮 (7 oxo DHEA)对该模型免疫系统功能的影响。方法 通过对BALB c小鼠连续 4周施加 7种应激原 ,建立慢性轻度应激动物模型。采用淋巴细胞增殖实验和NK细胞杀伤实验评价免疫功能的变化。结果 4周慢性应激处理后 ,小鼠T淋巴细胞对ConA刺激增殖反应、NK细胞杀伤活性及血清中T3和T4 水平明显下降。 7 oxo DHEA 15mg·kg- 1 能显著提高小鼠T淋巴细胞对ConA刺激增殖反应 ,并显著提高NK细胞杀伤活性及应激小鼠血清中T3和T4 浓度。结论 7 oxo DHEA能显著改善慢性应激引起的免疫功能低下。

Aim To determine whether 7-oxo-dehydroepiandrosterone (7-oxo-DHEA) can reverse the hypoimmunity in BALB/c mice exposed to chronic mild stress. Methods A chronic mild stress animal model was established by subjecting BALB/c mice to a stressful regimen arranged in an unpredicted manner for 4 consecutive weeks. Immunological function alternations under chronic mild stress were assessed by lymphocytes proliferative response to mitogens and NK cell lysis activity test. Results The studies showed the correlati...