微管失稳剂oncoprotein18/stathmin基因在深Ⅱ度烧伤愈合前创面及增生性瘢痕中的表达

Expression of microtubule destabilizer-oncoprotein18/stathmin gene in the hypertrophic scar before and after the healing of the deep partial thickness burn wounds

目的 观察深Ⅱ度烧伤创面愈合前及愈合后增生性瘢痕组织中微管失稳剂oncopro tein18 stathmin(Op18)基因的表达。 方法 提取临床深Ⅱ度烧伤愈合前与愈合后不同时期增生性瘢痕中的总RNA,以GADPH基因表达为内参照 ,采用逆转录酶 多聚酶链式反应 (RT PCR)方法 ,半定量检测上述基因表达水平。 结果 与正常组织比较 ,伤口愈合前Op18基因表达显著降低 (P <0.0 5 ),愈合后表达迅速增高 (P <0.0 5 ),在 4～ 32个月增生性瘢痕中Op18基因稳定高表达。 结论 在伤后早期Op18基因的表达水平降低与细胞增殖、组织修复的需要相适应 。

Objective To study the expression of microtubule destabilizer-oncoprotein18/stathmin(Op18) gene in the hypertrophic scar before and after the healing of the deep partial thickness burn wound. Methods Total RNA was extracted from the hypertrophic scar of the patients with deep partial thickness burn at different time before and after the wound healing. Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detect the expression of Op18 gene with GAPDH as an internal index. Results The expre...