牙鲆早期发育和变态期间Pitx2基因的表达(英文)

Gene expression of Pitx2 in early development and metamorphosis of Japanese flounder, Paralichthys olivaceus

Pitx2基因被认为是脊椎动物中调节内脏器官左右不对称的关键转录因子。为进一步探讨牙鲆变态期间的左右不对称是否也受Pitx2的左特异活性调节,采用逆转录聚合酶链式反应(RT-PCR),拟在变态的牙鲆的头部组织中克隆编码Pitx2基因的部分cDNA序列,并通过以18s rRNA为内标的半定量RT-PCR研究其在牙鲆早期发育和变态期间的表达情况。克隆和测序的结果表明Pitx2在牙鲆早期发育和变态期间也存在基因表达;而半定量的结果表明它的左特异性功能很可能在变态的早期阶段发挥作用。但是,牙鲆变态期间的右眼移动是否受Pitx2的左特异活性调控,以及变态期间的左右不对称和内脏器官的左右不对称是否拥有相同的分子机制,仍需进一步研究。

The gene Pitx2 is thought to be a critical transcriptional factor that mediates left-right asymmetry of internal organs in vertebrates. To demonstrate whether the metamorphic L/R asymmetry is regulated by the left-specific activity of Pitx2, cDNA of Pitx2 gene was amplified from the total RNA of head tissue of metamorphosing Japanese flounder, by reverse transcription polymerase chain reaction (RT-PCR), and different expression of Pitx2 was examined in developing flounder larvae by using relative quantitati...