一个人类线粒体转录终止样因子基因的克隆

The clone of a hunman mitochondrial transcription termination factor like

比较血清培养细胞和血清饥饿细胞的基因表达差异,获得了一段血清饥饿细胞中表达的cDNA序列,通过搜索表达序列标签(EST),拼接出完整的基因序列,通过PCR克隆获得全长cDNA序列.该基因全长1472bp,编码框预测有365个氨基酸.GenBank搜索,该基因与已有的人类线粒体转录终止因子有较大的同源性.所以,该基因可能是一个线粒体转录终止样因子.

Comparing the gene expression diference between normal cultured cells and serum starvation cells wigh DD-PCR method,a cDNA fragment,the full gene cDNA was obtained primarily by searching and splicing the EST sequences,then was cloned by PCR and sequenced.The size of this gene os 1472 bp and if codes apeptide containing 385 amino acid residues.It is homolgous with hunman mitochondrial transcription termination factor.So,this gene may be amitochondrial transcription termination factor like.