抗CD3单抗促进肿瘤细胞凋亡的研究

The correlation between apoptosis of tumor cells and anti-CD3McAb activated killer cells

目的 研究抗CD 3单抗活化T细胞后促进肿瘤细胞发生凋亡的作用。方法 应用流式细胞术并结合AnnexinⅤ FITC/PI双染色法检测细胞凋亡情况并对凋亡因子Fas抗原 (CD 95 )和Apo 2 .7进行定量分析。结果 加入抗CD 3单抗后可明显诱导肿瘤细胞的凋亡产生 (AnnexinⅤ FITC/PI染色 ) (P <0 .0 5 ) ,凋亡因子Fas抗原 (CD 95 )表达也明显增高 (P <0 .0 5 ) ,而Apo 2 .7仅略增高 (P >0 .0 5 )。结论 加入抗CD 3单抗活化的T细胞可明显促进肿瘤细胞发生凋亡 ;介导凋亡发生主要通过Fas抗原 (CD 95 )

Objective To investigate the correlation between apoptosis of tumor cells and anti CD3McAb activated killer cells. Methods We observed the apoptosis of tumor cells and the expression of CD 95 and Apo 2.7 by flow cytometry after affiliating into anti CD3McAb activated killer cells.Results After affiliating into anti CD3McAb activated killer cells, the apoptosis of tumor cells increased obviously( P <0.05) whereas the expression of CD 95 also increased significantly ( P <0.05).Conclusion Anti...