摘要
目的 研究木黄酮诱导膀胱癌T2 4细胞凋亡及与bcl 2基因表达的关系。方法 采用末端标记(TUNEL)法及流式细胞术 (FCM)研究T2 4细胞凋亡 ;采用FCM和RT PCR法检测bcl 2蛋白表达及mRNA表达水平的变化。结果 TUNEL法显示凋亡的T2 4细胞呈明显的形态学改变 ,FCM显示随木黄酮作用时间延长 ,T2 4细胞凋亡率逐渐增加 ,bcl 2蛋白表达逐渐下降 ;RT PCR法则显示bcl 2mRNA水平逐渐下降。结论 木黄酮能诱导T2 4细胞凋亡、下调bcl 2蛋白表达及bcl 2mRNA水平 ,且作用呈时间依赖性。
Objective To study the relationship between apoptosis induced by genistein in T24 cells and the expression of bcl 2 gene. Methods TUNEL(TdT mediated x dUTP nick end labeling) and FCM(flow cytometry) were used to investigate the apoptosis in T24 cells,and to observe the bcl 2 protein and bcl 2 mRNA by FCM and RT PCR assay. Results Apparent apoptotic morphological changes of T24 cells were observed by TUNEL. FCM and RT PCR assay indicated that the percentage of apoptic cells and the bcl 2 protein a...
出处
《上海医学》
CAS
CSCD
北大核心
2003年第S1期5-7,140,共4页
Shanghai Medical Journal
关键词
木黄酮
膀胱肿瘤
凋亡
Genistein
Bladder neoplasms
Apoptosis