补肾中药对溴隐亭致SD大鼠流产模型中的催乳素释放肽及其受体mRNA表达的影响

Effect of Bushen Herbs on Expression of Prolactin-Releasing Peptide and Its Receptor from the Improved Rat Model of Bromecriptine-Induced Abortion

目的:探讨补肾中药对溴隐亭致 SD 大鼠流产模型中胎盘、蜕膜催乳素释放肽(PrRP)的合成及其受体 mRNA 在垂体、胎盘、蜕膜表达的影响。方法:将 SD 孕大鼠随机分7组:其中 A～F组在孕6～8天皮下注射溴隐亭0.3mg·kg^(-1)·d^(-1),A 组为模型组,B、C、D 组孕1～11天分别灌服中药浸膏36g·kg^(-1)·d^(-1)、18g·kg^(-1)·d^(-1)I、中药粉剂3g·kg^(-)·d^(-1);E、F 组孕1～11天分别注射外源性催乳素(PRL)8 IU/次(每天2次)、黄体酮8mg·kg^(-1)·d^(-1);G 组为正常妊娠组。孕12天处死,取垂体、胎盘、蜕膜、用逆转录-聚合酶链反应(RT-PCR)方法测定各组大鼠垂体、胎盘、蜕膜催乳素释放肽受体(PrRP-R)的表达,观察蜕膜、胎盘 PrRP 的合成。结果:C、D、E、F 组的妊娠率与 A 组比较,差异有显著性(P<0.05,P<0.01),C、D 组的妊娠率与 E、F、G 组比较,差异无显著性。A 组垂体 PrRP-R 的表达明显高于其他各组(P<0.05),蜕膜、胎盘 PrRP-R 的表达各组无差异。仅 A 组内膜有 PrRP 合成。结论:与非孕期一样,孕期 PrRP 主要来源于下丘脑,胎盘与蜕膜很少有PrRP 的合成。蜕膜、垂体有 PrRP-R 的表达,补肾中药可能通过影响下丘脑合成 PrRP 从而促进垂体和蜕膜PRL 的释放。

Objective:To study the effect of Bushen herbs (BSH) on the expression of pr.lactin-releasing peptide (PrRP) from rat s pla- centa,decidue and its receptors from pituitary,placenta,decidua in the improved model of bromocriptine-induced abortion.Methods: Female SD rats were divided into 7 groups in random,group A was injected with bromocriptine (0.3mg/kg/d) during the pregnant day 6-8;injected with the same dosage as group A,group B and C were administered with extract of BSH(extractum) since preg- nancy (36g...