补肾益气活血方对宫内生长迟缓胎鼠肝脏C-Jun和C-Fos表达的影响

Effect of Bushen Yiqi Huoxue Recipe on Expression of C-Jun and C-Fos in Liver of Fetal with Intrauterine Growth Retardation

目的:探讨补肾益气活血方对胎儿宫内生长迟缓(intrauterine growth retardation,IUGR)胎鼠肝脏 C-Jun 和 C-Fos 表达的影响。方法:利用被动吸烟法建立 IUGR 大鼠模型,将动物分成正常组、IUGR 组、IUGR 中药治疗组(中药组)、IUGR 左旋精氨酸治疗组(精氨酸组),采用 Western-blot 方法检测各组肝脏 C-Jun 和 C-Fos 蛋白表达水平。结果:正常组胎鼠肝脏 C-Jun 有一定量表达,C-Fos 有少量表达;IUGR 组 C-Jun与 C-Fos 表达较正常组明显增强(P<0.05);中药组和精氨酸组 C-Jun 和 C-Fos 表达水平较 IUGR 组明显降低(P<0.05),且与正常组比较差异无显著性(P>0.05)。结论:IUGR 的发生与 C-Jun 和 C-Fos 表达改变有关,中药补肾益气活血方可能通过影响 C-Jun 和 C-Fos 表达而调节其他相关基因转录从而防治 IUGR。

Objective:To investigate the effect of Bushen Yiqi Huoxue Recipe (BSYQHX) on the expression of C-Jun and C-Fos in liver of fetal rats with intrauterine growth retardation (IUGR).Methods:The passive smoking was used to establish rat model of IUGR, which were divided into 4 groups:normal group,IUGR group,IUGR treated with TCM group (TCM group) and IUGR treated with L-arginine group (L-Arg group).Western Blot assay was used to measure the protein expression of C-Jun and C-Fos of fetal liver in the above-mentio...