RhoC基因在原发性肝癌中的表达及其意义

Influence of γ-Interferon on Expression of Fas and Bcl-2 of Liver Cancer Cell Line (Hep-G2)

目的:探讨原发性肝癌组织及癌旁肝组织中RhoC mRNA的表达及其意义。方法:采用RT-PCR技术分析30例原发性肝癌(HCC)患者的肝癌组织及癌旁肝组织中RhoC mRNA的表达。结果:肝癌组织中RhoC mRNA光密度相对值明显高于癌旁肝组织的光密度相对值,两者差异有显著性意义(P<0.01);有肝内转移灶者其癌组织中RhoC mRNA光密度相对值明显高于无肝内转移灶者(P<0.05)。结论:RhoC mRNA的表达与HCC的转移程度可能有关,可望作为估计HOC转移的参考指标。

Objective: To investigate the influence of γ-interferon (IFNg) cm the expression of Fas and Bcl-2 of liver cancer cell line (Hep-G2). Methods: Observing the expression of Fas and Bcl-2 by IFNg-pretreated Hep-G2 cells via immunohis-tochemical stain; Subsequendy treated these cells with Adriamycin, observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results: (1)IFNg up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P< 0.05). (2)The sensitivity of ...