RhoC基因及蛋白在胃癌肝转移中的表达及意义

Expression and Significance of RhoC mRNA and RhoC Protein in Human Gastric Carcinoma

目的:探讨胃癌肝转移中胃镜组织及肝转移癌组织中RhoC mRNA及蛋白的表达及意义。方法:采用逆转录聚合酶链式反应技术(RT-PCR)分析28例胃癌患者的癌组织(GC)及癌旁胃粘膜组织(PGMT)、肝转移癌(ML)组织中RhoC mRNA及蛋白的表达;采用免疫组织化学染色分析Rhoe蛋白的表达情况。结果:GC组织中RhoC mRNA光密度相对值为0.135±0.065;PGMT的光密度相对值为0.118±0.074,两者无显著性差异(P>0.05);ML中RhoC mRNA光密度相对值为0.641±0.113,与GC相比,差异有显著性意义(P<0.05)。RhoC蛋白的表达中,癌旁胃粘膜组织、胃癌组织、肝转移癌灶组织的灰度值分别为(128.523±11.486)U,(146.694±7.079)U,(162.351±14.612)U。经Student's t检验,差异有显著性意久(P<0.05);ML中RhoC mRNA及蛋白光密度相 对值明显高于GC(P<0.05)。结论:RhoC mRNA及蛋白的表达与胃癌转移有关,可用作判定胃癌转移及预后的指标。

Objective: To study the expression of RhoC mRNA and RhoC protein in human gastric carcinoma (GC), the paracarcinoma gastric mucosa tissues (PGMT), and metastasis of liver (ML). Methods: Reverse transcription polymerase chain reaction was used to detect the exprission of RhoC mRNA in the GC, PGMT and ML of 28 patient with gastric cancer. Results: The opacity density (A) of the expression of RhoC mRNA in GC was 0.135@0.065, not significantly higher than that of PGTM, which was 0.118@0.074 (P>0.05). But the...