小鼠生精干细胞的初步分离与纯化

Preliminary Isolation and Purification of Spermatogonial Stem Cells

目的 寻求一种获取小鼠的生精干细胞的简单方法。方法 将新生小鼠睾丸采用改良的两步消化法消化 ,并通过贴壁分离方法纯化。结果 通过该方法能够获取较高纯度 ( 95 % )的A型精原细胞 ,并通过生精干细胞移植实验证实其有效性。

Objective To obtain a simple method to isolate and purify spermatogonia stem cells. Methods The testis of newborn mouse were digested by modified two step digest method, and the type A spermatogonia cells were separated by different adhesive time with Sertoli cells. Results High concentration (95%) purified type A spermatogonia cells were acquired, and the spermatogonia transplanting experiment demonstrated the efficiency of the method.Conclusions This method is easy and efficient to isolation and purif...