非公平竞争对竞争抑制法检测抗-HBc总抗体的影响

Effects of the Unfair Competition on the Detection of Anti-HBc by Competitive Inhibition Method

目的:研究酶联免疫吸附实验(ELISA)在乙肝"两对半"中检测核心总抗体(抗HBc)总抗体由于加入标本与加入酶标记物在时间上不同步形成的"不公平"竞争对结果的影响。方法:收集单纯抗HBc阳性标本及部分阴性标本共90例,标本加入固相孔内放置不同时间后再加入抗HBc-辣根过氧化物酶(HRP)和将标本与抗HBc-HRP预先混匀后再加入固相孔内,所得结果与真实参考结果相比较,并同时考察由于加入标本与加入酶标记物在时间上不同步对乙肝表面抗原(HBsAg)阳性标本中抗-HBc的影响。结果:单纯抗HBc阳性标本加入标本与其后加入抗HBc-HRP的时间间隔越长假阳性越多,但这种影响对HBsAg阳性标本影响很小。结论:加入标本间隔一定时间后再加入抗HBc-HRP而引起的抗HBc假阳性,可通过在试管内预先混匀标本和抗HBc-HRP来消除。同时这种假阳性在HBsAg阳性标本中基本不存在。

Objective:To investigate the effects of the unfair competition on the detection of anti-HBc by competitive inhibition method.Methods:Ninety samples with positive and negative of anti-HBc were divided into two groups,namely experiment group and control group.The anti-HBc was detected by ELISA.In control group,total anti-HBc HRP were added following adding all of samples.In experiment group,the specimen was added after blending of samples and anti-HBc HRP.And then the results from two groups were compared.Mea...